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href="http://www.bmw97.com/Products-37031238.html" target="_blank"><strong>人細胞ELISA試劑盒</strong></a>的檢測過(guò)程大致如下：<br /> </div><div>　　1、包被：首先將針對目標抗原的特異性捕獲抗體固定在微孔板的各個(gè)孔內壁上，形成一層抗體膜。<br /> </div><div>　　2、封閉：為了避免非特異性結合，需要用封閉液填充未被捕獲抗體占據的位點(diǎn)。<br /> </div><div>　　3、加樣：將含有待測抗原的樣本加入到包被有捕獲抗體的孔中，抗原會(huì )與捕獲抗體特異性結合。<br /> </div><div>　　4、洗滌：去除未結合的物質(zhì)，通常使用洗滌緩沖液多次清洗。<br /> </div><div>　　5、加入檢測抗體：加入針對同一抗原的另一表位的酶標記檢測抗體，該抗體與抗原結合形成“夾心”復合物。<br /> </div><div>　　6、再次洗滌：去除未結合的檢測抗體。<br /> </div><div>　　7、顯色：加入酶的底物，酶標記的檢測抗體會(huì )催化底物發(fā)生化學(xué)反應，生成可見(jiàn)的顏色產(chǎn)物。<br /> </div><div>　　8、終止反應：根據實(shí)驗設計，可能需要加入終止液來(lái)停止顏色的發(fā)展。<br /> </div><div>　　9、讀數：使用酶標儀測量每個(gè)孔的光密度(OD值)，該值與樣本中抗原的濃度成正比。<br /> </div><div>　　通過(guò)與標準曲線(xiàn)比較，可以計算出樣本中抗原的濃度。ELISA試劑盒通常會(huì )提供一系列已知濃度的標準品，以建立標準曲線(xiàn)。<br /> </div><div>　　人細胞ELISA試劑盒的優(yōu)點(diǎn)包括靈敏度高、特異性強、操作相對簡(jiǎn)單、可同時(shí)處理大量樣本等。然而，ELISA結果的準確性和重復性受到多種因素的影響，包括抗體的質(zhì)量、樣本的處理和儲存條件、實(shí)驗操作的標準化等。因此，在進(jìn)行ELISA實(shí)驗時(shí)，需要嚴格按照說(shuō)明書(shū)的要求操作，并采取適當的質(zhì)量控制措施。</div> </div> </div> <div id="zbd1h1fdtj" class="cl"></div> <div id="zbd1h1fdtj" class="ye">上一條：沒(méi)有了<br />下一條：<a href="/News-1571058.html">白介素ELISA試劑盒的主要組成部分有哪些？</a></div> </div> </div> </div> <div id="zbd1h1fdtj" class="clearfix"></div> <div class="zbd1h1fdtj" id="clear"></div>  <footer class="footer"> <div id="zbd1h1fdtj" class="wrapper w1200"> <div id="zbd1h1fdtj" class="ft-inner"> <div id="zbd1h1fdtj" class="leftCon"> <div id="zbd1h1fdtj" class="ft-info"> <div id="zbd1h1fdtj" class="ft-logo"><img src="/Skins/529073/images/footlogo.png" alt="上?？婆嗳鹕锟萍加邢薰?></div> <div id="zbd1h1fdtj" class="ft-tel"> <span>郵箱</span> <p>2582166088@qq.com</p> </div> <div id="zbd1h1fdtj" class="ft-address"> <span>地址</span> <p>上海嘉定工業(yè)區葉城路912號J</p> </div> </div> </div> <div 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