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</br>適用范圍:實(shí)驗科研，不得用于臨床保質(zhì)期:6個(gè)月</br>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預先包被病毒性腹瀉病毒抗</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-30</li> <li><img src="/skins/529073/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>857</li> </ul> <div id="zbd1h1fdtj" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="zbd1h1fdtj" class="clear"></div> </div>  <div id="zbd1h1fdtj" class="pro_con" id="c_detail_wrap"> <div id="zbd1h1fdtj" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <section class="proshowParameter"> <table style=" margin-bottom:20px;"> <tbody> <tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫酶聯(lián)免疫</td><th>規格</th><td>48T/96T48T/96T</td></tr><tr><th>供貨周期</th><td>現貨</td><th>主要用途</th><td>用于科學(xué)實(shí)驗,不得用于臨床用于科學(xué)實(shí)驗,不得用于臨床</td></tr><tr><th>應用領(lǐng)域</th><td>醫療衛生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規格</th><td>48T/96T</td><th>儲存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢</th><td>靈敏性高、特異性強</td><th>特色服務(wù)</th><td>免費代測</td></tr> </tbody> </table> </section> <p><strong><strong>大鼠白介素1 IL-1 ELISA試劑盒</strong><br/>型號：KPR-DS11053<br/><strong>大鼠白介素1 IL-1 ELISA試劑盒</strong><br/>檢測試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫學(xué)診斷產(chǎn)品參數規格:48/96T 檢測方法:酶聯(lián)免疫品牌名稱(chēng):科培瑞儲存方式:2-8度冷藏適用范圍:實(shí)驗科研，不得用于臨床保質(zhì)期:6個(gè)月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標本、HRP標記的檢測抗原，經(jīng)過(guò)溫育并洗滌。用底物TMB顯色，TMB在過(guò)氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成終的黃色。用酶標儀在450NM波長(cháng)下測定吸光度(OD值)，與 CUTOFF值相比較，從而判定標本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><br/><br/><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過(guò)數年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營(yíng)為一體的專(zhuān)業(yè)化生物工程公司。公司具有具有完善的實(shí)驗技術(shù)開(kāi)發(fā)平臺，成熟的抗原、抗體研發(fā)系統，熟練掌握各種酶聯(lián)技術(shù)，結合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩定性和高的準確性，同時(shí)又具有競爭力的價(jià)格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實(shí)驗耗材等多門(mén)類(lèi)上萬(wàn)種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個(gè)領(lǐng)域。＃＃<br/></strong></p><p><strong>產(chǎn)品的穩定性如何？</strong></p><p><strong>產(chǎn)品在研發(fā)前期已通過(guò)嚴格的穩定測試，發(fā)貨前亦須通過(guò)檢測并質(zhì)檢，在市場(chǎng)上深受廣大客戶(hù)的贊許！</strong></p><p><strong>一次ELISA實(shí)驗需要多長(cháng)時(shí)間？</strong></p><p><strong>雙抗體夾心ELISA法一次實(shí)驗需要4-4.5小時(shí)，競爭ELISA法一次實(shí)驗需要2-2.5小時(shí)。</strong></p><p><strong><br/></strong></p><p><strong>二.方法類(lèi)型和操作步驟</strong></p><p><strong>雙位點(diǎn)一步法</strong></p><p><strong>在雙抗體夾心法測定抗原時(shí)，如應用針對抗原分子上兩個(gè)不同抗原決定簇的單克隆抗體分別作為固相抗體和酶標抗體，則在測定時(shí)可使標本的加入和酶標抗體的加入兩步并作一步（圖15-5）。這種雙位點(diǎn)一步不但簡(jiǎn)化了操作，縮短了反應時(shí)間，如應用高親和力的單克隆抗體，測定的敏感性和特異性也顯著(zhù)提高。單克隆抗體的應用使測定抗原的ELISA提高到新水平。</strong></p><p><strong>在一步法測定中，應注意鉤狀效應（hookeffect），類(lèi)同于沉淀反應中抗原過(guò)剩的后帶現象。當標本中待測抗原濃度相當高時(shí)，過(guò)量抗原分別和固相抗體及酶標抗體結合，而不再形成夾心復合物，所得結果將低于實(shí)際含量。鉤狀效應嚴重時(shí)甚至可出現假陰性結果。</strong></p><p><strong><br/>用途</strong></p><p><strong>ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性，酶標記的抗原或抗體既保留其免疫學(xué)活性，又保留酶的活性。在測定時(shí)，受檢標本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開(kāi)。再加入酶標記的抗原或抗體，也通過(guò)反應而結合在固相載體上。此時(shí)固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關(guān)，故可根據呈色的深淺進(jìn)行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應的結果，使測定方法達到很高的敏感度。 ELISA可用于測定抗原，也可用于測定抗體。然而，影響Elisa試驗結果的因素很多，故加強各個(gè)環(huán)節的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn)。</strong></p><p><strong><br/></strong></p><p></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p> </div> <div class="zbd1h1fdtj" id="order"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <a name="order" id="order"></a> <div id="zbd1h1fdtj" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="zbd1h1fdtj" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="大鼠白介素1 IL-1 ELISA試劑盒" placeholder="請輸入產(chǎn)品名稱(chēng)" /></div> </li> <li> <h4>您的單位：</h4> <div id="zbd1h1fdtj" class="msg_ipt12"><input class="textborder" size="42" name="department" id="department" placeholder="請輸入您的單位名稱(chēng)" /></div> </li> <li> <h4 class="xh">您的姓名：</h4> <div id="zbd1h1fdtj" class="msg_ipt1"><input class="textborder" size="16" name="yourname" id="yourname" 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